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Microscope Mounting Medium: Canada Balsam Dissolved in Benzene
Canada balsam is the oleoresin of the balsam fir, collected by breaking the blisters on its bark into a bottle (US Dispensatory, 1907). Dried, it is clear and glassy, with a refractive index close to crown glass, so it was long the standard medium for permanent microscope slides, dissolved in a solvent (Wikipedia). Here it is thickened and dissolved in benzene, which the Dispensatory names as dissolving it completely. Proportions are ours.
The picture: our drawing.
初学者
2 days
说明
1
1
Thicken the balsam
Thicken the balsam
Warm the balsam in an evaporating dish over a pan of hot water for some hours, stirring now and then, until a drop pulled out on the rod sets hard and clear when cold.
此步骤所需材料：
加拿大香脂100 克所需工具：
蒸发皿
高壁炖锅
电热板
玻璃搅拌棒2
2
Dissolve
Dissolve
Off the heat and away from any flame, stir in about its own weight of benzene until it is a clear, flowing syrup.
此步骤所需材料：
苯100 克所需工具：
玻璃搅拌棒
等臂天平
丁腈手套3
3
Filter and bottle
Filter and bottle
Filter it through paper into a clean bottle and keep it stoppered. If it thickens with time, thin it with a little more benzene.
所需工具：
带颈玻璃漏斗
滤纸
带塞玻璃瓶此蓝图制作
CC0 公共领域
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