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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C
The "mother seed" of oyster mushroom: wheat boiled, limed to about pH 9, sterilised in litre bottles and overgrown from a piece of agar culture. It is the spawn that starts a straw crop. The figures are those of a 2018 study at the Benemérita Universidad Autónoma de Puebla, Mexico, with their strain CP-50 of Pleurotus ostreatus.
Source: Valencia de Ita et al., J. Pure Appl. Microbiol. 12(2) 679-686 (2018), p. 680. The bottle closure, the scalpel and the hood are ours. The picture shows an oyster mushroom culture on agar, the starter.
中级
4 weeks
说明
1
1
Boil the wheat
Boil the wheat
Boil 10 kg of wheat in 15 L of water for 20 minutes. Let it stand 10 minutes, drain it and let it cool at room temperature for 30 minutes.
此步骤所需材料：
小麦10 公斤
水15 升所需工具：
高汤锅
滤盆
数字厨房秤2
2
Lime it
Lime it
Mix in 5 g of calcium hydroxide and 20 g of calcium sulfate evenly. They make the grain alkaline, about pH 9.
此步骤所需材料：
氢氧化钙（熟石灰）5 克
石膏（硫酸钙）20 克所需工具：
数字厨房秤高汤锅3
3
Bottle and sterilise
Bottle and sterilise
Pack the grain into 20 bottles of 1 L, close them, and sterilise them for 60 minutes at 121 °C. Leave them 24 hours to cool completely.
所需工具：
玻璃罐
台式高压灭菌器4
4
Inoculate from agar
Inoculate from agar
Working in a clean-air hood, cut a piece of agar colonised by the oyster mushroom with a flamed scalpel and drop it into each bottle. The study used 22.5 cm² per bottle.
此步骤所需材料：
蘑菇纯培养物20 个所需工具：
超净工作台
手术刀
酒精灯玻璃罐5
5
Incubate 25 days
Incubate 25 days
Keep the bottles at room temperature in indirect light, about 10 hours of light a day, for 25 days.
所需工具：
室温计玻璃罐此蓝图制作
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