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Microscope Mounting Medium: Canada Balsam Dissolved in Benzene
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Bob

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Bob

19. Oktoba 2026BE
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Microscope Mounting Medium: Canada Balsam Dissolved in Benzene

Canada balsam is the oleoresin of the balsam fir, collected by breaking the blisters on its bark into a bottle (US Dispensatory, 1907). Dried, it is clear and glassy, with a refractive index close to crown glass, so it was long the standard medium for permanent microscope slides, dissolved in a solvent (Wikipedia). Here it is thickened and dissolved in benzene, which the Dispensatory names as dissolving it completely. Proportions are ours. The picture: our drawing.
Mwanzo
2 days

Maagizo

1

Thicken the balsam

Warm the balsam in an evaporating dish over a pan of hot water for some hours, stirring now and then, until a drop pulled out on the rod sets hard and clear when cold.

Vifaa kwa hatua hii:

Zeri ya KanadaZeri ya Kanada100 g

Zana zinazohitajika:

Sahani ya KuvukiziaSahani ya Kuvukizia
Sufuria yenye kuta ndefuSufuria yenye kuta ndefu
Sahani ya Kupasha JotoSahani ya Kupasha Joto
Fimbo ya Kioo ya KukorogaFimbo ya Kioo ya Kukoroga
2

Dissolve

Off the heat and away from any flame, stir in about its own weight of benzene until it is a clear, flowing syrup.

Vifaa kwa hatua hii:

BenziniBenzini100 g

Zana zinazohitajika:

Fimbo ya Kioo ya KukorogaFimbo ya Kioo ya Kukoroga
Mizani ya mizani-mbiliMizani ya mizani-mbili
Glavu za NaitriliGlavu za Naitrili
3

Filter and bottle

Filter it through paper into a clean bottle and keep it stoppered. If it thickens with time, thin it with a little more benzene.

Zana zinazohitajika:

Faneli ya Kioo Yenye BombaFaneli ya Kioo Yenye Bomba
Karatasi ya KuchujaKaratasi ya Kuchuja
Chupa za kioo zenye viziboChupa za kioo zenye vizibo

Vifaa

2
  • 1
    Zeri ya Kanada
    100 g
    Kishikilia Nafasi
  • 2
    Benzini
    100 g
    Kishikilia Nafasi

Zana Zinazohitajika

9

Mchoro huu unatengeneza

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Kishikilia nafasi

Kiambatisho cha sampuli za hadubini

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Blueprint zinazohusiana

Blueprint hizi zinashiriki maarifa — mbinu, vifaa au kanuni

Making a Permanent Mounted Slide

Making a Permanent Mounted Slide

na KarenSE

Histolojia

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