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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C
Michał Wadas (Wikimedia Commons) · CC BY-SA 4.0
Bob

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Bob

26. Oktoba 2026BE
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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C

The "mother seed" of oyster mushroom: wheat boiled, limed to about pH 9, sterilised in litre bottles and overgrown from a piece of agar culture. It is the spawn that starts a straw crop. The figures are those of a 2018 study at the Benemérita Universidad Autónoma de Puebla, Mexico, with their strain CP-50 of Pleurotus ostreatus. Source: Valencia de Ita et al., J. Pure Appl. Microbiol. 12(2) 679-686 (2018), p. 680. The bottle closure, the scalpel and the hood are ours. The picture shows an oyster mushroom culture on agar, the starter.
Kati
4 weeks

Maagizo

1

Boil the wheat

Boil 10 kg of wheat in 15 L of water for 20 minutes. Let it stand 10 minutes, drain it and let it cool at room temperature for 30 minutes.

Vifaa kwa hatua hii:

NganoNgano10 kg
MajiMaji15 lita

Zana zinazohitajika:

Sufuria kubwa ya mchuziSufuria kubwa ya mchuzi
Kichujio chenye matunduKichujio chenye matundu
Mizani ya dijitali ya jikoniMizani ya dijitali ya jikoni
2

Lime it

Mix in 5 g of calcium hydroxide and 20 g of calcium sulfate evenly. They make the grain alkaline, about pH 9.

Vifaa kwa hatua hii:

Hidroksidi ya kalsiamu (chokaa iliyozimwa)Hidroksidi ya kalsiamu (chokaa iliyozimwa)5 g
Jipsamu (salfati ya kalsiamu)Jipsamu (salfati ya kalsiamu)20 g

Zana zinazohitajika:

Mizani ya dijitali ya jikoniMizani ya dijitali ya jikoni
Sufuria kubwa ya mchuziSufuria kubwa ya mchuzi
3

Bottle and sterilise

Pack the grain into 20 bottles of 1 L, close them, and sterilise them for 60 minutes at 121 °C. Leave them 24 hours to cool completely.

Zana zinazohitajika:

Chupa ya KiooChupa ya Kioo
Otoklavu ya mezaOtoklavu ya meza
4

Inoculate from agar

Working in a clean-air hood, cut a piece of agar colonised by the oyster mushroom with a flamed scalpel and drop it into each bottle. The study used 22.5 cm² per bottle.

Vifaa kwa hatua hii:

Utamaduni safi wa uyogaUtamaduni safi wa uyoga20 vipande

Zana zinazohitajika:

Kabati la mtiririko wa hewa sambambaKabati la mtiririko wa hewa sambamba
Kisu cha upasuajiKisu cha upasuaji
Taa ya spiriti (kichomea pombe)Taa ya spiriti (kichomea pombe)
Chupa ya KiooChupa ya Kioo
5

Incubate 25 days

Keep the bottles at room temperature in indirect light, about 10 hours of light a day, for 25 days.

Zana zinazohitajika:

Kipimajoto cha ChumbaniKipimajoto cha Chumbani
Chupa ya KiooChupa ya Kioo

Vifaa

5

Zana Zinazohitajika

9

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Blueprint zinazohusiana

Blueprint hizi zinashiriki maarifa — mbinu, vifaa au kanuni

Mushroom Grain Spawn: Sterile Wheat Overgrown with Mycelium, Sinden 1932

Mushroom Grain Spawn: Sterile Wheat Overgrown with Mycelium, Sinden 1932

na BobBE

Biolojia

8
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0
0
0
0
Pure Mushroom Culture: A Bit of Tissue in a Sterile Tube, Duggar 1905

Pure Mushroom Culture: A Bit of Tissue in a Sterile Tube, Duggar 1905

na BobBE

Biolojia

12
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CC0 Umma Wote

Mchoro huu umetolewa chini ya CC0. Uko huru kunakili, kubadilisha, kusambaza, na kutumia kazi hii kwa madhumuni yoyote, bila kuomba ruhusa.

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