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ẸWÀ ÀTI ÌLERA
IṢẸ́ ỌWỌ́
ÀṢÀ ÀTI ÌTÀN
ÌṢERÉ
ÀYÍKÁ
OÚNJẸ ÀTI OHUN MÍMU
REVERSE ENGINEERING
SÁYẸ́ǸSÌ
ERÉ ÌDÁRAYÁ
ÌMỌ̀-Ẹ̀RỌ
ÀWỌN OHUN WÍWỌ̀
n-Butanol and Acetone: Maize Mash Fermented by Weizmann's Bacteria
Weizmann's fermentation (US 1,315,585, 1919) turns maize starch into butyl alcohol and acetone. The bacteria live on the grain itself: you catch them by fermenting many small maize-mash cultures, keeping the ones that smell most strongly of butyl alcohol, and heating each subculture to 90–100 °C for a minute or two so that only their heat-proof spores survive. The finished culture ferments a sterilised mash of 100 parts maize meal in 1500 of water in about two days, and the mash is then distilled.
The picture: our diagram.
Ilọsíwájú
About 2 days a batch, once the culture is raised
Ìlànà
1
1
Start many cultures
Start many cultures
Make about 100 tubes of hot (90–100 °C), dilute (about 2 %), sterile maize mash, add a little raw maize meal to each, and let them ferment at about 35–37 °C for four to five days.
Àwọn irinṣẹ́ tí a nílò:
Ìyẹ̀fun àgbàdo
Omi
Àwọn tube ìdánwò
Autoclave orí tábìlì
Ìwẹ̀ Omi
Ẹ̀rọ Ìwọ̀n Ooru Yàrá Ìwádìí2
2
Pick the butanol smellers
Pick the butanol smellers
Choose the tubes that ferment most vigorously and smell most strongly of butyl alcohol. Heat them to 90–100 °C for one to two minutes: most bacteria die, but the wanted heat-resisting spores survive.
Àwọn irinṣẹ́ tí a nílò:
Àwọn tube ìdánwòÌwẹ̀ OmiẸ̀rọ Ìwọ̀n Ooru Yàrá Ìwádìí
Ìbọ̀wọ́ Tí Ń Fara Da Ooru3
3
Subculture 100 to 150 times
Subculture 100 to 150 times
Inoculate a fresh sterilised maize mash with the heated culture. When it has grown, heat it to 90–100 °C for one to two minutes and use it to inoculate the next mash. Repeat 100 to 150 times. Air need not be kept out.
Àwọn irinṣẹ́ tí a nílò:
Ìyẹ̀fun àgbàdoOmiÀwọn tube ìdánwòAutoclave orí tábìlìÌwẹ̀ OmiẸ̀rọ Ìwọ̀n Ooru Yàrá Ìwádìí4
4
Make and sterilise the mash
Make and sterilise the mash
Mix 100 parts maize meal with 1500 parts water. Sterilise it for three to four hours at 130–140 °C (2–3 atmospheres) and cool it.
Àwọn ohun èlò fún ìgbésẹ̀ yìí:
Ìyẹ̀fun àgbàdo100 partsÀwọn irinṣẹ́ tí a nílò:
Omi
Òǹwọ̀n ìdọ́gbaAutoclave orí tábìlìÌbọ̀wọ́ Tí Ń Fara Da Ooru5
5
Ferment two days
Ferment two days
Inoculate with the final culture and keep at about 35–36 °C. Fermentation starts in five to ten hours, runs strongly for about 36 hours and is complete in about 48. It works open to the air or in closed tanks.
Àwọn irinṣẹ́ tí a nílò:
Ohun èlò ìwúkàrà (ìgò gíláàsì ńlá)Ẹ̀rọ Ìwọ̀n Ooru Yàrá Ìwádìí6
6
Distil off butanol and acetone
Distil off butanol and acetone
Distil the mash and separate the products by fractional distillation into acetone and butyl alcohol.
Àwọn irinṣẹ́ tí a nílò:
Ohun ìsun tí ó pínẸ̀rọ Ìwọ̀n Ooru Yàrá ÌwádìíÀwọn ohun-èlò
1- 100 partsÀyè
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