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Mushroom Grain Spawn: Sterile Wheat Overgrown with Mycelium, Sinden 1932
Spawn used to be composted, washed manure packed into bricks, or into bottles that had to be smashed to get it out. James Sinden of Penn State patented spawn on cooked, sterilised grain instead. Every grain ends up coated in mycelium, the spawn shakes out of its jar, and it can be sown in rows like seed.
Source: J. W. Sinden, US 1,869,517 (1932), written for the field mushroom (Agaricus). The starter is a pure culture, here from the tissue-culture blueprint.
Katamtaman
3 weeks
Mga Tagubilin
1
1
Wet the wheat
Wet the wheat
Put wheat in a jar with just enough water for the grain to swell as it cooks and leave no excess. You can also use excess water and pour it off after sterilising. Sinden prefers the first way.
Mga materyales para sa hakbang na ito:
Trigo1 as required
Tubig1 as requiredMga kailangang kasangkapan:
Garapong Salamin2
2
Plug and sterilise
Plug and sterilise
Stopper the jar with cotton so it stays sterile after steaming. Steam it at 15 to 20 lb pressure for no longer than an hour. The cooked grain is then about 71 % water.
Mga kailangang kasangkapan:
Bulak na sumisipsip
Autoclave na pang-mesa3
3
Inoculate
Inoculate
When the jar is cool, put in a bit of pure mycelium. A few grains shaken from an earlier grain culture also work, with no instrument needed.
Mga materyales para sa hakbang na ito:
Purong kultura ng kabute1 as requiredMga kailangang kasangkapan:
Garapong Salamin4
4
Incubate and shake
Incubate and shake
Keep the jar at 20 to 25 °C until the mycelium has overgrown the grain. Shake it now and then. Shaking spreads the mycelium and keeps the grains separate.
Mga kailangang kasangkapan:
Termometro ng SilidGarapong Salamin5
5
Shake it out and sow
Shake it out and sow
The spawn is ready when each grain is covered with a mat of mycelium. Shake it out and break up any lumps. Sow it in rows like radish or carrot seed, or in hills, 2 inches (5 cm) deep in the compost.
Mga kailangang kasangkapan:
Garapong SalaminMga Materyales
3- 1 as requiredPlaceholder
- 1 as requiredPlaceholder
- 1 as requiredPlaceholder
Mga Kinakailangang Kasangkapan
4- Placeholder
- Placeholder
- Placeholder
- Placeholder
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