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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C
English
Bob

Yaremwe na

Bob

26. Ukwakira 2026BE
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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C

The "mother seed" of oyster mushroom: wheat boiled, limed to about pH 9, sterilised in litre bottles and overgrown from a piece of agar culture. It is the spawn that starts a straw crop. The figures are those of a 2018 study at the Benemérita Universidad Autónoma de Puebla, Mexico, with their strain CP-50 of Pleurotus ostreatus. Source: Valencia de Ita et al., J. Pure Appl. Microbiol. 12(2) 679-686 (2018), p. 680. The bottle closure, the scalpel and the hood are ours. The picture shows an oyster mushroom culture on agar, the starter.
Hagati
4 weeks

Amabwiriza

1

Boil the wheat

Boil 10 kg of wheat in 15 L of water for 20 minutes. Let it stand 10 minutes, drain it and let it cool at room temperature for 30 minutes.

Ibikoresho by'iyi ntambwe:

InganoIngano10 kg
AmaziAmazi15 litiro

Ibikoresho bikenewe:

Inkono Nini y'UmutobeInkono Nini y'Umutobe
Akayungurizo gafite imyoboAkayungurizo gafite imyobo
Iminzani ya Digitale yo mu GikoniIminzani ya Digitale yo mu Gikoni
2

Lime it

Mix in 5 g of calcium hydroxide and 20 g of calcium sulfate evenly. They make the grain alkaline, about pH 9.

Ibikoresho by'iyi ntambwe:

Hidorogiside ya kalisiyumu (ishwagara yazimye)Hidorogiside ya kalisiyumu (ishwagara yazimye)5 g
Jipise (sulufate ya kalisiyumu)Jipise (sulufate ya kalisiyumu)20 g

Ibikoresho bikenewe:

Iminzani ya Digitale yo mu GikoniIminzani ya Digitale yo mu Gikoni
Inkono Nini y'UmutobeInkono Nini y'Umutobe
3

Bottle and sterilise

Pack the grain into 20 bottles of 1 L, close them, and sterilise them for 60 minutes at 121 °C. Leave them 24 hours to cool completely.

Ibikoresho bikenewe:

Icupa ry'IkirahureIcupa ry'Ikirahure
Otokilave yo ku mezaOtokilave yo ku meza
4

Inoculate from agar

Working in a clean-air hood, cut a piece of agar colonised by the oyster mushroom with a flamed scalpel and drop it into each bottle. The study used 22.5 cm² per bottle.

Ibikoresho by'iyi ntambwe:

Umuco usukuye w'ibihumyoUmuco usukuye w'ibihumyo20 ibice

Ibikoresho bikenewe:

Akabati k’umwuka utembera kimweAkabati k’umwuka utembera kimwe
Icyuma cyo kubagaIcyuma cyo kubaga
Itara rya alcoolItara rya alcool
Icupa ry'IkirahureIcupa ry'Ikirahure
5

Incubate 25 days

Keep the bottles at room temperature in indirect light, about 10 hours of light a day, for 25 days.

Ibikoresho bikenewe:

Igipimo cy'ubushyuhe cy'icyumbaIgipimo cy'ubushyuhe cy'icyumba
Icupa ry'IkirahureIcupa ry'Ikirahure

Ibikoresho

5

Ibikoresho bikenewe

9

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