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Microscope Mounting Medium: Canada Balsam Dissolved in Benzene
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19. uMfumfu 2026BE
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Microscope Mounting Medium: Canada Balsam Dissolved in Benzene

Canada balsam is the oleoresin of the balsam fir, collected by breaking the blisters on its bark into a bottle (US Dispensatory, 1907). Dried, it is clear and glassy, with a refractive index close to crown glass, so it was long the standard medium for permanent microscope slides, dissolved in a solvent (Wikipedia). Here it is thickened and dissolved in benzene, which the Dispensatory names as dissolving it completely. Proportions are ours. The picture: our drawing.
Oqalayo
2 days

Imiyalelo

1

Thicken the balsam

Warm the balsam in an evaporating dish over a pan of hot water for some hours, stirring now and then, until a drop pulled out on the rod sets hard and clear when cold.

Izinto zokwakha zalesi sinyathelo:

I-balsam yase-CanadaI-balsam yase-Canada100 g

Amathuluzi adingekayo:

Ipuleti LokuhwamukaIpuleti Lokuhwamuka
Ibhodwe elinezindonga eziphakemeIbhodwe elinezindonga eziphakeme
Ipuleti ElishisayoIpuleti Elishisayo
Induku Yengilazi YokugovuzaInduku Yengilazi Yokugovuza
2

Dissolve

Off the heat and away from any flame, stir in about its own weight of benzene until it is a clear, flowing syrup.

Izinto zokwakha zalesi sinyathelo:

I-benzeneI-benzene100 g

Amathuluzi adingekayo:

Induku Yengilazi YokugovuzaInduku Yengilazi Yokugovuza
Isikali sesilinganisoIsikali sesilinganiso
Amagilavu E-nitrileAmagilavu E-nitrile
3

Filter and bottle

Filter it through paper into a clean bottle and keep it stoppered. If it thickens with time, thin it with a little more benzene.

Amathuluzi adingekayo:

Ifanela Lengilazi ElinomlomoIfanela Lengilazi Elinomlomo
Iphepha LokuhluzaIphepha Lokuhluza
Amabhodlela engilazi anezivimboAmabhodlela engilazi anezivimbo

Izinto

2

Amathuluzi Adingekayo

9

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Making a Permanent Mounted Slide

Making a Permanent Mounted Slide

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Histology

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