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Mushroom Grain Spawn: Sterile Wheat Overgrown with Mycelium, Sinden 1932
English
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26. uMfumfu 2026BE
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Mushroom Grain Spawn: Sterile Wheat Overgrown with Mycelium, Sinden 1932

Spawn used to be composted, washed manure packed into bricks, or into bottles that had to be smashed to get it out. James Sinden of Penn State patented spawn on cooked, sterilised grain instead. Every grain ends up coated in mycelium, the spawn shakes out of its jar, and it can be sown in rows like seed. Source: J. W. Sinden, US 1,869,517 (1932), written for the field mushroom (Agaricus). The starter is a pure culture, here from the tissue-culture blueprint.
Ophakathi
3 weeks

Imiyalelo

1

Wet the wheat

Put wheat in a jar with just enough water for the grain to swell as it cooks and leave no excess. You can also use excess water and pour it off after sterilising. Sinden prefers the first way.

Izinto zokwakha zalesi sinyathelo:

UkolweniUkolweni1 as required
AmanziAmanzi1 as required

Amathuluzi adingekayo:

Ijeke LengilaziIjeke Lengilazi
2

Plug and sterilise

Stopper the jar with cotton so it stays sterile after steaming. Steam it at 15 to 20 lb pressure for no longer than an hour. The cooked grain is then about 71 % water.

Amathuluzi adingekayo:

Ukotini omuncayoUkotini omuncayo
I-autoclave yetafulaI-autoclave yetafula
3

Inoculate

When the jar is cool, put in a bit of pure mycelium. A few grains shaken from an earlier grain culture also work, with no instrument needed.

Izinto zokwakha zalesi sinyathelo:

Isiko lamakhowe esihlanzekileIsiko lamakhowe esihlanzekile1 as required

Amathuluzi adingekayo:

Ijeke LengilaziIjeke Lengilazi
4

Incubate and shake

Keep the jar at 20 to 25 °C until the mycelium has overgrown the grain. Shake it now and then. Shaking spreads the mycelium and keeps the grains separate.

Amathuluzi adingekayo:

Ithemomitha YekameloIthemomitha Yekamelo
Ijeke LengilaziIjeke Lengilazi
5

Shake it out and sow

The spawn is ready when each grain is covered with a mat of mycelium. Shake it out and break up any lumps. Sow it in rows like radish or carrot seed, or in hills, 2 inches (5 cm) deep in the compost.

Amathuluzi adingekayo:

Ijeke LengilaziIjeke Lengilazi

Izinto

3

Amathuluzi Adingekayo

4

CC0 Isizinda Somphakathi

Le blueprint ikhishwe ngaphansi kwe-CC0. Ukhululekile ukukopisha, ukuguqula, ukusabalalisa, nokusebenzisa ngaphandle kwemvume.

Sekela uMenzi ngokuthenga imikhiqizo nge-Blueprint yabo IKhomishane Yomenzi kumiswe ngabathengisi, noma dala inguqulo entsha yale Blueprint bese uyifaka njengoxhumaniso ku-Blueprint yakho ukuze wabelane ngemali engenayo.

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