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Microscope Slides Cut from Sheet, Coverslips from a Blown Bubble
A microscope slide is a strip of plain glass about 75 × 25 mm and 1 mm thick with smoothed edges; a coverslip is a much thinner square that presses the specimen flat. Cut the slides from thin sheet and grind the edges; for coverslips, blow a thin bubble on a glass tube and cut flat pieces from it.
The picture: a tray of early 20th-century prepared slides under coverslips, museum of the Institut Pasteur de Lille.
中级
An afternoon
说明
1
1
Cut the slides
Cut the slides
Mark thin glass sheet in 25 mm strips, score and snap them, then score and snap each strip at 75 mm.
此步骤所需材料:
玻璃板1 个所需工具:
玻璃刀
直尺
安全眼镜2
2
Grind the edges
Grind the edges
Rub every edge and corner on a flat glass plate with wet fine grit until smooth to the touch. Wash and dry.
此步骤所需材料:
水1 升所需工具:
6 英寸数显卡尺3
3
Blow a thin bubble
Blow a thin bubble
Close the end of a glass tube in the torch, heat it evenly and blow a large, very thin bubble, turning it as you blow (our method).
此步骤所需材料:
玻璃管套件1 个所需工具:
台式玻璃吹制喷灯
安全眼镜
耐热手套4
4
Cut coverslips
Cut coverslips
When cold, break the bubble and pick the flattest pieces from its sides. Trim them to small squares with the cutter and check the thickness with the caliper; keep only even, thin pieces.
所需工具:
玻璃刀
6 英寸数显卡尺
安全眼镜5
5
Store clean
Store clean
Wash slides and coverslips and keep them apart in a closed jar so they stay free of dust and grease.
此步骤所需材料:
水1 升所需工具:
玻璃罐6
6
Slides from a sheet
Slides from a sheet
正在加载 Jupyter 笔记本…
7
7
Limits
Limits
Slide size and edge finish are as Wikipedia gives them; the blown-bubble coverslips are our method. Blown coverslips vary in thickness: measure them, since the wrong thickness blurs high-power images.
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