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Microscope Slides Cut from Sheet, Coverslips from a Blown Bubble
A microscope slide is a strip of plain glass about 75 × 25 mm and 1 mm thick with smoothed edges; a coverslip is a much thinner square that presses the specimen flat. Cut the slides from thin sheet and grind the edges; for coverslips, blow a thin bubble on a glass tube and cut flat pieces from it.
The picture: a tray of early 20th-century prepared slides under coverslips, museum of the Institut Pasteur de Lille.
Kati
An afternoon
Maagizo
1
1
Cut the slides
Cut the slides
Mark thin glass sheet in 25 mm strips, score and snap them, then score and snap each strip at 75 mm.
Vifaa kwa hatua hii:
Bamba la kioo1 kipandeZana zinazohitajika:
Kikata kioo
Rula
Miwani ya usalama2
2
Grind the edges
Grind the edges
Rub every edge and corner on a flat glass plate with wet fine grit until smooth to the touch. Wash and dry.
Vifaa kwa hatua hii:
Maji1 litaZana zinazohitajika:
Kipima-unene cha dijitali cha inchi 6
Chembechembe za kabidi ya silikoni3
3
Blow a thin bubble
Blow a thin bubble
Close the end of a glass tube in the torch, heat it evenly and blow a large, very thin bubble, turning it as you blow (our method).
Vifaa kwa hatua hii:
Seti ya Mirija ya Kioo1 kipandeZana zinazohitajika:
Kichomeo cha mezani cha kupuliza kiooMiwani ya usalama
Glavu Zinazostahimili Joto4
4
Cut coverslips
Cut coverslips
When cold, break the bubble and pick the flattest pieces from its sides. Trim them to small squares with the cutter and check the thickness with the caliper; keep only even, thin pieces.
Zana zinazohitajika:
Kikata kiooKipima-unene cha dijitali cha inchi 6Miwani ya usalama5
5
Store clean
Store clean
Wash slides and coverslips and keep them apart in a closed jar so they stay free of dust and grease.
Vifaa kwa hatua hii:
Maji1 litaZana zinazohitajika:
Chupa ya Kioo6
6
Slides from a sheet
Slides from a sheet
Inapakia daftari la Jupyter…
7
7
Limits
Limits
Slide size and edge finish are as Wikipedia gives them; the blown-bubble coverslips are our method. Blown coverslips vary in thickness: measure them, since the wrong thickness blurs high-power images.
Vifaa
3- 1 kipandeKishikilia Nafasi
- 2 litaKishikilia Nafasi
- 1 kipandeKishikilia Nafasi
Zana Zinazohitajika
8- Kishikilia Nafasi
- Kishikilia Nafasi
- Kishikilia Nafasi
- Kishikilia Nafasi
- Kishikilia Nafasi
- Kishikilia Nafasi
- Kishikilia Nafasi
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