សិល្បៈ
សម្រស់ និង សុខុមាលភាព
សិប្បកម្ម
វប្បធម៌ និង ប្រវត្តិសាស្ត្រ
ការកម្សាន្ត
បរិស្ថាន
ម្ហូប និង ភេសជ្ជៈ
វិស្វកម្មបញ្ច្រាស
វិទ្យាសាស្ត្រ
កីឡា
បច្ចេកវិទ្យា
ប្រដាប់ដែលស្លៀក
Making a Permanent Mounted Slide
A drop of pond water under a coverslip is a wet mount, and it has about twenty minutes to live. The water creeps out, the coverslip drifts, air pushes in from the edges, and whatever you found is gone before you can show it to anyone.
A permanent mount replaces the water with a resin that sets clear and hard, sealing the specimen between glass forever. Victorian slide-makers turned this into an industry — there are mounted diatom arrangements from the 1870s that are still perfect — and the chemistry has barely changed.
The reason it works is not just preservation. The mounting medium is chosen to have a refractive index close to glass, so the specimen stops scattering light at every boundary and detail that was lost in glare simply appears. A good mount makes a specimen LOOK better, not only last longer.
ចាប់ផ្តើម
3 hours
ការណែនាំ
1
1
Clean the glass properly
Clean the glass properly
Wash slides and coverslips in detergent, rinse, dip in alcohol and dry with a lint-free cloth. Handle them by the edges only.
Grease makes the mountant pull away into islands as it dries, leaving a permanently ruined slide that looks fine while it is wet. A fingerprint at 400× is a landscape. This is the same lesson as cleaning a TLC plate, and it fails the same way: silently, later, and only after you have done all the other work.
សម្ភារៈសម្រាប់ជំហាននេះ៖
បន្ទះកញ្ចក់មីក្រូទស្សន៍និងបន្ទះគម្រប20 ដុំ
អេតាណុល - ថ្នាក់មន្ទីរពិសោធន៍, 95 %100 មលឧបករណ៍ដែលត្រូវការ៖
ស្រោមដៃនីទ្រីល2
2
Fix the specimen so it stops changing
Fix the specimen so it stops changing
Soak the specimen in a fixative — formalin, or a simple alcohol-acetic acid mixture — for long enough to reach the middle of it. Thin material needs minutes, thick material needs hours.
Fixing cross-links the proteins so the tissue stops decaying and holds its shape through everything that follows. Skip it and your beautiful section turns to mush in the alcohol. Work with formalin only in good ventilation and gloves — the vapour is an irritant and a sensitiser, and an alcohol-acetic fixative is perfectly adequate for plant material.
សម្ភារៈសម្រាប់ជំហាននេះ៖
សូលុយស្យុងហ្វម៉ាល់ដេអ៊ីត (ហ្វម៉ាលីន ៣៧ %)50 មលអេតាណុល - ថ្នាក់មន្ទីរពិសោធន៍, 95 %200 មល
អាស៊ីតអាសេទិក (ក្លាស្យាល)20 មលឧបករណ៍ដែលត្រូវការ៖
ដបកញ្ចក់ស្រោមដៃនីទ្រីល
វ៉ែនតាសុវត្ថិភាពបិទជិត3
3
Dehydrate in steps, never in one jump
Dehydrate in steps, never in one jump
Move the specimen up a series of alcohol baths — 30, 50, 70, 90 and finally 100 per cent — giving it time in each.
Water and resin do not mix, so every trace of water must go before mounting. But dropping wet tissue straight into absolute alcohol pulls water out so fast that cells shrink and distort, and delicate structures collapse. The graded series is slow and it is the difference between a section that looks like tissue and one that looks like a raisin.
សម្ភារៈសម្រាប់ជំហាននេះ៖
អេតាណុល - ថ្នាក់មន្ទីរពិសោធន៍, 95 %500 មលឧបករណ៍ដែលត្រូវការ៖
ដបកញ្ចក់
នាឡិកាបញ្ឈប់4
4
Mount without trapping air
Mount without trapping air
Put a single small drop of mountant on the slide, lower the coverslip from one edge at a shallow angle, and let it fall the rest of the way under its own weight.
Dropping it flat traps a sheet of bubbles that you will never get out. Lowering from one edge pushes the air ahead of the advancing liquid. Use less mountant than feels right: too much oozes out and takes weeks to harden, too little pulls air in from the edges as it shrinks. One small drop for a standard coverslip.
សម្ភារៈសម្រាប់ជំហាននេះ៖
សារធាតុភ្ជាប់សម្រាប់មីក្រូទស្សន៍10 មលឧបករណ៍ដែលត្រូវការ៖
ពីប៉ែតមានខ្នាតវាស់ (ម៉ោរ)5
5
Dry it flat, and label it immediately
Dry it flat, and label it immediately
Lay the slide flat, somewhere warm and dust-free, for days to weeks depending on the mountant. Write the label as soon as the coverslip is down.
Tilted slides let the coverslip creep and the specimen ends up off-centre or half out. And an unlabelled slide is worthless within a month — you will not remember what it was, where it came from or how it was stained. Species or material, source, date, fixative, stain: five fields, thirty seconds, and the slide stays useful for a century.
6
6
Why the mountant makes things visible
Why the mountant makes things visible
កំពុងផ្ទុកសៀវភៅ Jupyter…
សម្ភារៈសម្រាប់ជំហាននេះ៖
គ្លីសេរីន (ពីរុក្ខជាតិ)50 មល7
7
Ring the semi-permanent mounts
Ring the semi-permanent mounts
For a glycerol or water mount, paint a ring of clear nail varnish around the coverslip edge in two thin coats, letting the first dry.
The ring does two jobs: it stops evaporation and it glues the coverslip down so an objective cannot drag it. Two thin coats beat one thick one, which shrinks as it dries and pulls away from the glass. Victorian mounters made this decorative — coloured rings and gilt paper — and the practical version is exactly the same thing without the flourish.
សម្ភារៈ
6- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
ឧបករណ៍ចាំបាច់
5- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
- កន្លែងទុក
ប្លង់ពាក់ព័ន្ធ
ប្លង់ទាំងនេះចែករំលែកចំណេះដឹង — បច្ចេកទេស សម្ភារៈ ឬគោលការណ៍
CC0 សាធារណៈ
ប្លង់នេះត្រូវបានចេញផ្សាយក្រោម CC0។ អ្នកមានសិទ្ធិចម្លង កែប្រែ ចែកចាយ និងប្រើប្រាស់ដោយមិនចាំបាច់សុំអនុញ្ញាត។
គាំទ្រអ្នកបង្កើតដោយទិញផលិតផលតាមរយៈប្លង់របស់ពួកគេ ដែលពួកគេទទួលបាន កម្រៃជើងសារអ្នកបង្កើត កំណត់ដោយអ្នកលក់ ឬបង្កើតកំណែថ្មីនៃប្លង់នេះ ហើយបញ្ចូលជាការតភ្ជាប់ក្នុងប្លង់របស់អ្នកដើម្បីចែករំលែកចំណូល។