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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C
The "mother seed" of oyster mushroom: wheat boiled, limed to about pH 9, sterilised in litre bottles and overgrown from a piece of agar culture. It is the spawn that starts a straw crop. The figures are those of a 2018 study at the Benemérita Universidad Autónoma de Puebla, Mexico, with their strain CP-50 of Pleurotus ostreatus.
Source: Valencia de Ita et al., J. Pure Appl. Microbiol. 12(2) 679-686 (2018), p. 680. The bottle closure, the scalpel and the hood are ours. The picture shows an oyster mushroom culture on agar, the starter.
Àárín
4 weeks
Ìlànà
1
1
Boil the wheat
Boil the wheat
Boil 10 kg of wheat in 15 L of water for 20 minutes. Let it stand 10 minutes, drain it and let it cool at room temperature for 30 minutes.
Àwọn ohun èlò fún ìgbésẹ̀ yìí:
Àlìkámà10 kg
Omi15 lítàÀwọn irinṣẹ́ tí a nílò:
Ìkòkò Ọbẹ̀ Ńlá
Àgbọ̀n ìṣẹ́ oníhò
Òṣùwọ̀n Díjítà Ilé Ìdáná2
2
Lime it
Lime it
Mix in 5 g of calcium hydroxide and 20 g of calcium sulfate evenly. They make the grain alkaline, about pH 9.
Àwọn ohun èlò fún ìgbésẹ̀ yìí:
Calcium hydroxide (ẹfun tí a pa)5 g
Gypsum (calcium sulfate)20 gÀwọn irinṣẹ́ tí a nílò:
Òṣùwọ̀n Díjítà Ilé Ìdáná
Ìkòkò Ọbẹ̀ Ńlá3
3
Bottle and sterilise
Bottle and sterilise
Pack the grain into 20 bottles of 1 L, close them, and sterilise them for 60 minutes at 121 °C. Leave them 24 hours to cool completely.
Àwọn irinṣẹ́ tí a nílò:
Ìgò Gílásì
Autoclave orí tábìlì4
4
Inoculate from agar
Inoculate from agar
Working in a clean-air hood, cut a piece of agar colonised by the oyster mushroom with a flamed scalpel and drop it into each bottle. The study used 22.5 cm² per bottle.
Àwọn ohun èlò fún ìgbésẹ̀ yìí:
Àgbìn olú mímọ́20 ẹyọÀwọn irinṣẹ́ tí a nílò:
Kọ́bọ́ọ̀dù afẹ́fẹ́ tí ń ṣàn lọ́gbọọgba
Abẹ iṣẹ́-abẹ
Fìtílà ọtí (fìtílà spirit)
Ìgò Gílásì5
5
Incubate 25 days
Incubate 25 days
Keep the bottles at room temperature in indirect light, about 10 hours of light a day, for 25 days.
Àwọn irinṣẹ́ tí a nílò:
Ìwọ̀n Ooru Yàrá
Ìgò GílásìÀwọn ohun-èlò
5- 10 kgÀyè
- 15 lítàÀyè
- Àyè
- 20 ẹyọÀyè
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