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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C
English
Bob

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Bob

26. uMfumfu 2026BE
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Oyster Mushroom Grain Spawn: Boiled Wheat with Lime and Gypsum, 121 °C

The "mother seed" of oyster mushroom: wheat boiled, limed to about pH 9, sterilised in litre bottles and overgrown from a piece of agar culture. It is the spawn that starts a straw crop. The figures are those of a 2018 study at the Benemérita Universidad Autónoma de Puebla, Mexico, with their strain CP-50 of Pleurotus ostreatus. Source: Valencia de Ita et al., J. Pure Appl. Microbiol. 12(2) 679-686 (2018), p. 680. The bottle closure, the scalpel and the hood are ours. The picture shows an oyster mushroom culture on agar, the starter.
Ophakathi
4 weeks

Imiyalelo

1

Boil the wheat

Boil 10 kg of wheat in 15 L of water for 20 minutes. Let it stand 10 minutes, drain it and let it cool at room temperature for 30 minutes.

Izinto zokwakha zalesi sinyathelo:

UkolweniUkolweni10 kg
AmanziAmanzi15 amalitha

Amathuluzi adingekayo:

Ibhodwe Elikhulu LomhluziIbhodwe Elikhulu Lomhluzi
Isihluzi esinezimboboIsihluzi esinezimbobo
Isikali Sedijithali SasekhishiniIsikali Sedijithali Sasekhishini
2

Lime it

Mix in 5 g of calcium hydroxide and 20 g of calcium sulfate evenly. They make the grain alkaline, about pH 9.

Izinto zokwakha zalesi sinyathelo:

I-calcium hydroxide (umcako ocishiwe)I-calcium hydroxide (umcako ocishiwe)5 g
I-gypsum (i-calcium sulfate)I-gypsum (i-calcium sulfate)20 g

Amathuluzi adingekayo:

Isikali Sedijithali SasekhishiniIsikali Sedijithali Sasekhishini
Ibhodwe Elikhulu LomhluziIbhodwe Elikhulu Lomhluzi
3

Bottle and sterilise

Pack the grain into 20 bottles of 1 L, close them, and sterilise them for 60 minutes at 121 °C. Leave them 24 hours to cool completely.

Amathuluzi adingekayo:

Ijeke LengilaziIjeke Lengilazi
I-autoclave yetafulaI-autoclave yetafula
4

Inoculate from agar

Working in a clean-air hood, cut a piece of agar colonised by the oyster mushroom with a flamed scalpel and drop it into each bottle. The study used 22.5 cm² per bottle.

Izinto zokwakha zalesi sinyathelo:

Isiko lamakhowe esihlanzekileIsiko lamakhowe esihlanzekile20 izicucu

Amathuluzi adingekayo:

Ikhabethe lomoya ogeleza ngokulinganayoIkhabethe lomoya ogeleza ngokulinganayo
I-scalpelI-scalpel
Isibani sikagologoIsibani sikagologo
Ijeke LengilaziIjeke Lengilazi
5

Incubate 25 days

Keep the bottles at room temperature in indirect light, about 10 hours of light a day, for 25 days.

Amathuluzi adingekayo:

Ithemomitha YekameloIthemomitha Yekamelo
Ijeke LengilaziIjeke Lengilazi

Izinto

5

Amathuluzi Adingekayo

9

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